[A comparison of methodologic approaches to the study of amylase in the blood]

The blood serum alpha-amylase activity has been measured in normal male subjects by three methods, i. e. amyloclastic (W. Caraway's), with the use of the chromogenic insoluble substrate, and saccharogenic enzymic technique. The enzymic method has proved to be the most accurate and reproducible, whereas the amyloclastic one the least so. The enzyme activity values determined by the three methods are in poorly manifest though reliable correlation. The findings suggest that the data of the methods based on measurements of various parameters of the enzyme are not fully compatible.