Epithelial sodium channel modulates platelet collagen activation
Doris Cerecedo1, Ivette Martínez-Vieyra1, Lea Alonso-Rangel1
1Laboratorio de Hematobiología, Escuela Nacional de Medicina y Homeopatía (ENMH), Instituto Politécnico Nacional (IPN), Mexico City, Mexico.
European Journal of Cell Biology
|April 1, 2014
Summary
The Epithelial sodium channel (ENaC) is not essential for platelet migration or granule release. However, sodium influx via ENaC is crucial for platelet activation by collagen.
Area of Science:
- Platelet biology
- Ion channel function
- Hemostasis
Background:
- Platelet activation involves calcium influx and cytoskeletal changes.
- The Epithelial sodium channel (ENaC) regulates sodium transport and is involved in various physiological processes.
- ENaC's role in platelet function, particularly in migration and granule secretion, is not well understood.
Purpose of the Study:
- To investigate the expression, distribution, and functional role of ENaC in human platelets.
- To determine ENaC's involvement in platelet migration, granule secretion, and collagen-induced activation.
Main Methods:
- Biochemical analysis and confocal microscopy to study ENaC localization in platelets.
- Pharmacological inhibition of ENaC using amiloride.
- Platelet migration assays.
- Quantification of soluble P-selectin and serotonin release.
Main Results:
- ENaC is expressed in platelets and associates with intermediate filaments and dystrophin-associated proteins.
- ENaC is dispensable for platelet migration and alpha/dense granule secretion.
- Sodium influx through ENaC is essential for collagen-induced platelet activation.
Conclusions:
- ENaC plays a critical role in mediating sodium influx required for collagen-stimulated platelet activation.
- ENaC is not required for platelet shape change, migration, or general granule release.
- ENaC represents a potential therapeutic target for modulating platelet hyperreactivity.
Keywords:
Adhered plateletsAmiloride (AMR)Dystrophin-associated proteins (DAP)Epithelial sodium channel (ENaC)Intermediate filaments (IF)Platelet migrationMore Related Videos
09:13Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
Published on: April 6, 2017
11.3K
04:37Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation
Published on: May 23, 2025
1.2K
Related Concept Videos
01:22Formation of the Platelet Plug
6.2K
The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
6.2K
01:17Intracellular Signaling Affects Focal Adhesions
2.8K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
2.8K
01:18Structure and Function of Platelets
7.1K
The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
7.1K
01:20Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors
1.6K
Antiplatelet drugs emerge as frontline defenders against the insidious threat of thromboembolic diseases, where abnormal clots obstruct vital blood vessels. These drugs stand as bulwarks, inhibiting platelet aggregation and clot formation, thereby mitigating the risk of life-threatening conditions like myocardial infarction, coronary artery disease, and thrombotic strokes.
Prostaglandin synthesis inhibitors, exemplified by the widely known aspirin, wield their power by irreversibly acetylating...
Prostaglandin synthesis inhibitors, exemplified by the widely known aspirin, wield their power by irreversibly acetylating...
1.6K
01:21Paracrine Signaling
50.3K
Paracrine signaling allows cells to communicate with their immediate neighbors via secretion of signaling molecules. Such a signal can only trigger a response in nearby target cells because the signal molecules degrade quickly or are inactivated if not taken up. Prominent examples of paracrine signaling include nitric oxide signaling in blood vessels, synaptic signaling of neurons, the blood clotting system, tissue repair/wound healing, and local allergic skin reactions. Nitric oxide as a...
50.3K
01:56Structural Protein Function
28.9K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
28.9K