Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

Mouse Models of Cancer Study

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

BAd-looking or CHarming: The two faces of BACH2.

The Journal of experimental medicine·2026

Same author

Antibody Blockade of Ly49/MHC-I interactions enhances Innate and Adaptive Immunity Against Cancer Metastasis.

bioRxiv : the preprint server for biology·2026

Same author

Modulating protein corona effects in SERS detection with inorganic salts: a strategy for improved melamine detection in milk.

Food chemistry·2026

Same author

Integrated metagenomic and culturomic strategies to mine and validate beneficial rhizosphere <i>Actinobacteria</i> from lavender.

Frontiers in plant science·2026

Same author

Bacterial Community Structure and FEAST Source Tracking of Endophytes in <i>Vernonia anthelmintica</i> (L.) Willd. from Southern Xinjiang, China.

Microorganisms·2026

Same author

Screening of Lipid-Lowering Acid Bacteria from Traditional Koumiss to Develop a New Probiotic Starter Culture.

Probiotics and antimicrobial proteins·2026

Search research articles

Related Experiment Video

Updated: May 1, 2026

Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord

Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord

Published on: March 18, 2013

Immunologic applications of conditional gene modification technology in the mouse.

Suveena Sharma¹, Jinfang Zhu¹

¹Molecular and Cellular Immunoregulation Unit, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.

Current Protocols in Immunology

|April 5, 2014

Summary

The Cre-loxP system combined with homologous recombination enables advanced mouse models for immunology research. This technology allows for conditional gene knockout and lineage tracing to study immune cell development and function.

Keywords:

Cre bacterial artificial chromosome (BAC)conditional knockout fate mapping hematopoietic cells inducible gene expression loxP lymphocyte cell development and differentiation transgenic

More Related Videos

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection

Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection

Published on: February 2, 2018

Related Experiment Videos

Last Updated: May 1, 2026

Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord

Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord

Published on: March 18, 2013

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection

Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection

Published on: February 2, 2018

Area of Science:

Immunology
Genetics
Molecular Biology

Background:

Homologous recombination and the Cre-loxP system are powerful tools for genome manipulation.
These technologies have been instrumental in creating sophisticated mouse models for biological research.

Purpose of the Study:

To summarize the principles and applications of Cre-loxP technology in mouse immunology.
To discuss the utility of Cre-expressing mouse lines for studying lymphocyte development and function.
To highlight potential challenges and considerations for data interpretation using these models.

Main Methods:

Review of Cre-loxP system principles and applications.
Discussion of commonly used Cre-expressing mouse lines.
Analysis of factors affecting gene deletion efficiency and Cre activity.

Main Results:

Conditional gene knockout, inducible/tissue-specific gene overexpression, and lineage fate mapping are key applications.
Various Cre-expressing mouse lines offer distinct advantages for lymphocyte studies.
Efficiency of gene deletion, Cre leakiness, and Cre toxicity can impact experimental outcomes.

Conclusions:

Cre-loxP technology is vital for dissecting immune cell development and responses in mice.
Careful selection of mouse lines and awareness of potential technical issues are crucial for reliable results.
This review provides a foundation for utilizing and interpreting data from Cre-loxP-based mouse models in immunology.