[Comparative analysis of methods of evaluating antibody-dependent cell-mediated cytotoxicity]

Two methods for measuring the activity of antibody-dependent cell-mediated cytotoxicity are compared. One of them is based on the determination of the relative amount of hemoglobin released from lysed sheep target red cells into the incubation medium via benzidine hydrochloride, that joins hemoglobin in the presence of H2O2 and stains the solution blue, the intensity of staining being measured spectrophotometrically. The authors consider radiometry preferable; this method makes use of 3H uridine-labeled chicken nuclear red cells as targets, and the results of the test are analyzed ina beta-scintillator. Less patient's blood is necessary for this test, since lower concentrations of effector cells may be used.