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A switchable peptide sensor for real-time lysosomal tracking.

Lei Chen1, Junchen Wu, Carsten Schmuck

  • 1Key Labs for Advanced Materials and Institute of Fine Chemicals, East China University of Science and Technology, Shanghai 200237, China. jcwu@ecust.edu.cn tianhe@ecust.edu.cn.

Chemical Communications (Cambridge, England)
|April 10, 2014
PubMed
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A novel peptide probe enables real-time tracking of lysosomes within cells. This biocompatible tool offers reversible monitoring for cellular research.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Imaging

Background:

  • Lysosomes are crucial organelles involved in cellular degradation and waste removal.
  • Accurate tracking of lysosomal dynamics is essential for understanding cellular processes and disease mechanisms.

Purpose of the Study:

  • To develop a novel peptide-based probe for efficient and reversible lysosomal tracking.
  • To evaluate the cell-permeability, biocompatibility, and cytotoxicity of the developed probe.

Main Methods:

  • Synthesis of a bis-spiropyran functionalized peptide (peptide 1).
  • Assessment of cell-permeability using live-cell imaging.
  • Evaluation of biocompatibility and cytotoxicity through standard assays.
  • Demonstration of reversible lysosomal tracking capabilities.

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Last Updated: May 1, 2026

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Main Results:

  • Peptide 1 demonstrated good cell-permeability, allowing effective entry into cells.
  • The probe exhibited excellent biocompatibility and low cytotoxicity, ensuring minimal impact on cellular functions.
  • Reversible and real-time tracking of lysosomes was successfully achieved using peptide 1.

Conclusions:

  • The developed bis-spiropyran functionalized peptide 1 is a promising tool for lysosomal tracking.
  • Its favorable properties make it suitable for real-time and reversible monitoring of lysosomal activity in live cells.
  • This probe can advance research in cellular biology and related disease mechanisms.