Related Experiment Video
Updated: Jul 25, 2026
11:17
Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses
Published on: August 30, 2018
[A stat-method of determining antibiotic concentration in biological fluids]
Laboratornoe Delo
|January 1, 1989
Summary
A new rapid method accurately measures antibiotic levels in biologic samples by assessing Staphylococcus aureus dehydrogenase activity. This 4-hour test shows high correlation with standard methods, offering a simpler, faster alternative.
Area of Science:
- Microbiology
- Biochemistry
- Analytical Chemistry
Context:
- Accurate measurement of antibiotic concentrations is crucial for effective treatment and preventing resistance.
- Existing methods for antibiotic quantification can be time-consuming and resource-intensive.
Purpose:
- To develop and validate a rapid, simple, and cost-effective method for determining antibiotic concentrations in biologic substrates.
Summary:
- A novel assay utilizes the suppression of dehydrogenase activity in Staphylococcus aureus 209 P to quantify antibiotic levels.
- Results from this rapid method demonstrate a high correlation (r = 0.84-0.93, p < 0.001) with the standard agar diffusion test.
- The technique provides results within 4 hours, requiring less nutrient media.
Impact:
- This rapid assay offers a significant improvement in turnaround time for antibiotic level determination.
- The method's simplicity and reduced media requirements make it suitable for clinical and research settings.
- Potential to optimize antibiotic dosing and improve patient outcomes.
More Related Videos
Related Concept Videos
01:23Drug Concentrations: Measurements
Drug concentration is the quantity of a drug present in a biological sample. Measuring drug amounts in biological samples allows the clinician to understand how a drug is absorbed, distributed, metabolized, and excreted. Samples can be obtained through invasive or non-invasive methods. Invasive techniques involve surgical or parenteral interventions to gather blood, cerebrospinal fluid, or tissue biopsy. Conversely, non-invasive approaches provide samples like urine, feces, and saliva.
Plasma —...
Plasma —...
01:15Determination of Multiple Dosing Parameters: Steady-State, Minimum and Maximum Concentrations
Gentamicin, an aminoglycoside antibiotic, is commonly administered via intermittent intravenous infusion to treat severe infections. An intermittent one-hour infusion of gentamicin, administered at eight-hour intervals, allows for precise control of plasma drug concentrations, minimizing toxicity while ensuring therapeutic efficacy. Pharmacokinetic principles govern the dynamics of plasma concentrations and can be mathematically described using specific equations.The plasma drug concentration...
01:24Automated Microbial Diagnostics
Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...