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Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
Enhanced phytate dephosphorylation by using Candida melibiosica yeast-based biofuel cell
Yolina Hubenova1, Danail Georgiev, Mario Mitov
1Department of Biochemistry and Microbiology, "Paisii Hilendarski" University of Plovdiv, 24 Tzar Asen Str., 4000, Plovdiv, Bulgaria, jolinahubenova@yahoo.com.
Abstract:
We report for the first time that Candida melibiosica expresses enhanced phytase activity when grown under biofuel cell polarization in a nutrient-poor medium, containing only fructose as a carbohydrate source. Phytase activity during the cultivation under polarization reached up to 25 U per g dry biomass, exceeding with 20 ± 3 % those of the control. A participation of the enzyme in the adaptation processes to the stress conditions is proposed. In addition, steady-state electrical outputs were achieved during biofuel cell operation at continuous polarization under constant load. The obtained results show that C. melibiosica yeast-based biofuel cell could be used for simultaneous electricity generation and phytate bioremediation.
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