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Updated: Apr 28, 2026
Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
Published on: January 30, 2016
A new field-of-view autotracking method for online tomography reconstruction based on back-projected ray image
Sachihiko Tomonaga1, Misuzu Baba2, Sadao Yamazaki3
1Major of Informatics, Graduate School, Kogakuin University, 2665-1 Nakano, Hachioji, Tokyo 192-0015, Japan.
Abstract:
We devised a new field-of-view autotracking method for online tomography reconstruction based on a cross-correlation between a pair of neighbours, called back-projected ray images, among a specimen tilt sequence. One ray image is calculated through normal filtered back-projection only in the cross-sectional plane from each projection image. This ray-image matching can reliably track the field-of-view because a pair of neighbouring ray images mostly cross-correlates at the existing three-dimensional object position. Online experiments using real specimens resulted in successful autotracking performance with high accuracy, and online tomograms were obtained immediately after the final tracking at the last tilting angle.
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