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Erythritol production on wheat straw using Trichoderma reesei.
Birgit Jovanović1, Robert L Mach1, Astrid R Mach-Aigner1
1Department for Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna University of Technology, Gumpendorfer Str. 1a, A-1060 Wien, Austria.
AMB Express
|June 21, 2014
Summary
Overexpressing the err1 gene in Trichoderma reesei enhanced erythritol production, particularly in the Rut-C30 strain using lignocellulosic substrates like wheat straw.
Area of Science:
- Biotechnology
- Industrial Microbiology
- Metabolic Engineering
Background:
- Trichoderma reesei is a fungus known for its cellulase production capabilities.
- Erythritol is a sugar alcohol with various industrial applications.
- Enhancing erythritol production in T. reesei could offer a sustainable bioprocess.
Purpose of the Study:
- To investigate the feasibility of erythritol production in Trichoderma reesei.
- To evaluate the overexpression of the err1 gene for enhanced erythritol yield.
- To assess production using both D-xylose and pretreated lignocellulosic substrates.
Main Methods:
- Overexpression of the err1 gene in wild-type and Rut-C30 T. reesei strains using constitutive (pki) and inducible (bxl1) promoters.
- Characterization of err1 transcript formation on D-xylose and xylan.
- Erythritol production experiments in shake flasks and bioreactors.
- Utilization of alkaline organosolve pretreated wheat straw as a lignocellulosic substrate.
Main Results:
- Shake flask experiments showed increased erythritol formation in both wild-type and Rut-C30 strains overexpressing err1 compared to parental strains.
- Bioreactor cultivation on wheat straw did not enhance erythritol production in the wild-type overexpression strain.
- Overexpression of err1 in the Rut-C30 strain significantly increased erythritol formation on wheat straw.
Conclusions:
- The err1 gene is a viable target for enhancing erythritol production in T. reesei.
- The Rut-C30 strain, a cellulase hyperproducer, shows superior performance for erythritol synthesis when err1 is overexpressed, especially on lignocellulosic materials.
- This study demonstrates the potential of engineered T. reesei for sustainable erythritol production from agricultural waste.
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