Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Radical Autoxidation01:20

Radical Autoxidation

2.5K
The oxidation of an organic compound in the presence of air or oxygen is called autoxidation. For example, cumene reacts with oxygen to form hydroperoxide. Autoxidation involves initiation, propagation, and termination steps. Many organic compounds are susceptible to autoxidation—especially ethers in the presence of oxygen, which form hydroperoxides. Even though this reaction is slow, old ether bottles contain small amounts of peroxide, which leads to laboratory explosions during ether...
2.5K
Lipid-derived Compounds in the Human Body01:31

Lipid-derived Compounds in the Human Body

5.5K
Fats and lipids are crucial components in the human body. Some lipid-derived compounds, such as fat-soluble vitamins, eicosanoids, lipoproteins, and glycolipids, also play unique roles to support various  biological processes .
Fat-soluble Vitamins
Fat-soluble vitamins, including vitamins A, D, E, and K, are required in minimal quantities, but their deficiencies can lead to severely abnormal physiological conditions. For example, vitamin A deficiency can cause night blindness, dry skin,...
5.5K
Peroxisomes01:24

Peroxisomes

13.6K
Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
13.6K
Structure of Lipids03:38

Structure of Lipids

87.6K
Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic...
87.6K
Atherosclerosis III: Management01:26

Atherosclerosis III: Management

719
Management of atherosclerosis involves an integrated strategy encompassing pharmacological treatment, surgical interventions, lifestyle changes, and nutrition therapy to address the multifactorial nature of the disease.Pharmacological TherapyA cornerstone of atherosclerosis management is the use of pharmacological agents. Statins, such as atorvastatin, are pivotal in inhibiting HMG-CoA reductase, an enzyme that catalyzes an initial step in cholesterol synthesis in the liver. This reduction in...
719
Lipids: Dietary Sources and Requirements01:18

Lipids: Dietary Sources and Requirements

2.4K
Lipids are an essential component of a balanced human diet. Triglycerides, which make up the majority of dietary lipids, are found in both saturated fats—commonly present in meat, dairy products, and certain tropical plants like coconut, and hydrogenated oils such as margarine and baking shortenings (trans fats)—and unsaturated fats, which are abundant in seeds, nuts, olive oil, and most vegetable oils. The main sources of cholesterol include egg yolks, various meats and organ...
2.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Lightweight and Low-Voltage-Operating Linear Actuator Based on the Electroactive Polymer Polypyrrole.

Polymers·2023
Same author

Significance of Singlet Oxygen Molecule in Pathologies.

International journal of molecular sciences·2023
Same author

A novel electrochemical biosensing method with double-layered polymer brush modified electrode.

Colloids and surfaces. B, Biointerfaces·2022
Same author

Positive Association between Aqueous Humor Hydroxylinoleate Levels and Intraocular Pressure.

Molecules (Basel, Switzerland)·2022
Same author

Singlet oxygen -derived nerve growth factor exacerbates airway hyperresponsiveness in a mouse model of asthma with mixed inflammation.

Allergology international : official journal of the Japanese Society of Allergology·2022
Same author

<i>In Situ</i> Electrical Monitoring of Methylated DNA Based on Its Conformational Change to G-Quadruplex Using a Solution-Gated Field-Effect Transistor.

Analytical chemistry·2021

Related Experiment Video

Updated: Apr 26, 2026

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
07:29

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Published on: October 12, 2017

8.4K

Do n-3 polyunsaturated fatty acids increase or decrease lipid peroxidation in humans?

Nirvair S Kelley1, Yasukazu Yoshida, Kent L Erickson

  • 11 Arizona College of Osteopathic Medicine, Midwestern University , Glendale, Arizona.

Metabolic Syndrome and Related Disorders
|July 22, 2014
PubMed
Summary

Omega-3 polyunsaturated fatty acids (PUFA) supplementation does not appear to increase lipid peroxidation (LPO) based on a review of human studies. Methodological differences in studies significantly impact results regarding n-3 PUFA and LPO markers.

More Related Videos

Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

3.0K
DNBS/TNBS Colitis Models: Providing Insights Into Inflammatory Bowel Disease and Effects of Dietary Fat
09:04

DNBS/TNBS Colitis Models: Providing Insights Into Inflammatory Bowel Disease and Effects of Dietary Fat

Published on: February 27, 2014

47.6K

Related Experiment Videos

Last Updated: Apr 26, 2026

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
07:29

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Published on: October 12, 2017

8.4K
Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

3.0K
DNBS/TNBS Colitis Models: Providing Insights Into Inflammatory Bowel Disease and Effects of Dietary Fat
09:04

DNBS/TNBS Colitis Models: Providing Insights Into Inflammatory Bowel Disease and Effects of Dietary Fat

Published on: February 27, 2014

47.6K

Area of Science:

  • Biochemistry
  • Nutrition Science
  • Oxidative Stress Research

Background:

  • Omega-3 polyunsaturated fatty acids (PUFA) offer known health benefits.
  • Concerns exist that high unsaturation in n-3 PUFA may elevate oxidative stress and lipid peroxidation (LPO).

Purpose of the Study:

  • To review human studies on the effects of n-3 PUFA supplementation on lipid peroxidation markers.
  • To assess the overall impact of n-3 PUFA on oxidative stress indicators.

Main Methods:

  • Analysis of results from 22 published human studies.
  • Comparison of studies based on methodology, subject characteristics, dosage, duration, and supplement composition.
  • Focus on methods for measuring lipid peroxidation (LPO) markers.

Main Results:

  • Inconsistencies in LPO marker changes across studies (9 no change, 8 decrease, 5 increase).
  • Studies measuring in vivo F2-isoprostanes or plasma antioxidant capacity were more likely to show decreased LPO.
  • Methods involving ex vivo sample preparation or oxidation (e.g., malondialdehyde) were associated with increased or unchanged LPO markers.

Conclusions:

  • The majority of studies suggest n-3 PUFA do not increase lipid peroxidation (LPO).
  • Methodological variations are a key factor in observed inconsistencies.
  • Future research requires standardized diets and methods to clarify n-3 PUFA effects on LPO products.