Related Experiment Video
Updated: Nov 10, 2025
07:15
Transient Optical Clearing Using Absorbing Molecules for Ex Vivo and In Vivo Imaging
Published on: July 11, 2025
1.0K
Viscous optical clearing agent for in vivo optical imaging
Zijian Deng1, Lijia Jing2, Ning Wu1
1Peking University, College of Engineering, Department of Biomedical Engineering, No. 5 Yiheyuan Road, Haidian, Beijing 100871, China.
Journal of Biomedical Optics
|July 29, 2014
Summary
A new viscous optical clearing agent (OCA) improves deep tissue imaging by allowing more light penetration. This high-viscosity OCA overcomes limitations of fluid agents for in vivo studies on uneven surfaces.
Area of Science:
- Biomedical Optics
- Medical Imaging
- Materials Science
Background:
- Optical clearing agents (OCAs) enhance light penetration for deeper tissue imaging.
- Conventional OCAs' high fluidity limits in vivo applications on dynamic or irregular surfaces.
Purpose of the Study:
- To develop and evaluate a novel, high-viscosity optical clearing agent.
- To assess the efficacy of the viscous OCA in improving in vivo optical imaging.
Main Methods:
- Characterization of the viscous OCA's physical and optical properties.
- In vivo imaging of living animal skin using photoacoustic microscopy and optical coherence tomography with the viscous OCA.
Main Results:
- The viscous OCA was successfully synthesized and characterized.
- Demonstrated effective performance in enhancing image quality and depth in living skin using both photoacoustic microscopy and optical coherence tomography.
- The agent's viscosity prevented displacement on uneven and moving surfaces.
Conclusions:
- The developed viscous OCA offers a significant advancement over traditional fluid agents.
- This high-viscosity OCA shows great potential for various in vivo optical imaging modalities in turbid tissues.
- Addresses limitations of current OCAs for challenging in vivo imaging scenarios.
