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Multiple GO-SELEX for efficient screening of flexible aptamers
Van-Thuan Nguyen1, Young Seop Kwon, Jae Hoon Kim
1School of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-Gu, Seoul 136-713, Republic of Korea. mbgu@korea.ac.kr.
Chemical Communications (Cambridge, England)
|July 30, 2014
Summary
We developed a fast aptamer screening method using graphene oxide for small molecules. This technique identified specific and flexible multi-target aptamers for pesticides, useful for biosensors.
Area of Science:
- Biochemistry
- Materials Science
- Analytical Chemistry
Background:
- Aptamer selection (SELEX) typically requires target immobilization, which can be challenging for small molecules.
- High-throughput screening methods are needed for efficient discovery of aptamers against diverse targets.
Purpose of the Study:
- To develop a simple, high-speed, and high-throughput aptamer screening method for small molecules.
- To identify aptamers capable of binding to single or multiple small molecule targets, including pesticides.
Main Methods:
- A novel aptamer screening method, simple Multi-GO-SELEX, was employed using graphene oxide without target immobilization.
- The method was applied to screen for aptamers against three different pesticide molecules.
Main Results:
- Ten single-stranded DNA (ssDNA) aptamers were successfully generated for the target pesticides.
- Aptamer affinities ranged from 10-100 nM.
- Several flexible multi-target aptamers were identified, capable of binding to two or three different pesticide molecules.
Conclusions:
- The simple Multi-GO-SELEX method provides an efficient platform for screening aptamers against small molecules.
- The identified flexible multi-target aptamers are promising for simultaneous detection and biosensor applications.
