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Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia
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STING-dependent cytosolic DNA sensor pathways regulate NKG2D ligand expression.

Nina Le Bert1, Adeline R Lam2, Samantha Sw Ho1

  • 1Immunology Programme; Centre of Life Sciences; Department of Microbiology; Yong Loo Lin School of Medicine; National University of Singapore; Singapore.

Oncoimmunology
|August 13, 2014
PubMed
Summary

The DNA damage response (DDR) in lymphoma cells triggers cytosolic DNA, activating STING pathways. This leads to increased RAE-1 expression, a ligand for NKG2D, enhancing immune responses.

Keywords:
DNA sensorNKG2DNatural killer cellssignal transductiontumor immunology

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Area of Science:

  • Immunology
  • Molecular Biology
  • Cancer Research

Background:

  • The DNA damage response (DDR) is known to upregulate NKG2D ligand (NKG2DL) expression.
  • Recent findings indicate the DDR also induces cytosolic DNA in B-cell lymphoma.

Purpose of the Study:

  • To investigate the link between DDR-induced cytosolic DNA and NKG2D ligand expression in B-cell lymphoma.
  • To elucidate the role of STING-dependent pathways in this process.

Main Methods:

  • Analysis of DNA damage response pathways in B-cell lymphoma models.
  • Detection of cytosolic DNA and assessment of STING pathway activation.
  • Measurement of RAE-1 ligand expression.

Main Results:

  • The DDR in B-cell lymphoma cells leads to the presence of cytosolic DNA.
  • Activation of STING-dependent cytosolic DNA sensor pathways was observed.
  • This activation resulted in the expression of RAE-1, a ligand for NKG2D.

Conclusions:

  • DDR-induced cytosolic DNA activates STING pathways in B-cell lymphoma.
  • This pathway promotes RAE-1 expression, a key NKG2D ligand, potentially influencing anti-tumor immunity.