Related Experiment Video
Updated: Apr 24, 2026
09:11
Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors
Published on: November 23, 2019
6.4K
A new substrate for alkaline phosphatase based on quercetin pentaphosphate
Samuel K Mwilu1, Veronica A Okello, Francis J Osonga
1Department of Chemistry, Center for Advanced Sensors & Environmental Monitoring (CASE), State University of New York-Binghamton, P. O. Box 6000, Binghamton, NY 13902, USA. osadik@binghamton.edu.
The Analyst
|September 3, 2014
Summary
A new fluorimetric substrate, quercetin pentaphosphate (QPP), effectively detects alkaline phosphatase (ALP) activity. QPP offers high sensitivity and stability, making it a valuable tool for ALP detection in various applications.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Enzyme Assays
Background:
- Alkaline phosphatase (ALP) is a crucial enzyme in various biological processes.
- Sensitive and stable substrates are needed for accurate ALP activity detection.
- Existing fluorimetric substrates have limitations in stability or sensitivity.
Purpose of the Study:
- To characterize a novel fluorimetric substrate, quercetin pentaphosphate (QPP).
- To evaluate QPP's efficacy and stability for alkaline phosphatase (ALP) detection.
- To compare QPP with existing fluorescent substrates for ALP assays.
Main Methods:
- Characterization of QPP's spectral properties (absorbance and fluorescence).
- Quantification of ALP activity using QPP via absorbance and fluorescence measurements.
- Assessment of QPP stability in solution and under storage conditions.
- Application of QPP for detecting Bacillus globigii spores.
Main Results:
- QPP exhibits distinct spectral properties suitable for enzymatic detection.
- ALP activity detection using QPP showed linear ranges and low limits of detection (0.766 U L⁻¹ by absorbance, 0.062 U L⁻¹ by fluorescence).
- QPP demonstrated excellent stability (over 3.5 months in solution, 12 months storage) and detected Bacillus globigii spores (limit 5998 spores/mL).
Conclusions:
- Quercetin pentaphosphate (QPP) is a highly effective and stable fluorimetric substrate for alkaline phosphatase (ALP) detection.
- QPP offers comparable or superior performance to existing fluorescent substrates.
- QPP's stability and sensitivity make it a promising tool for biochemical and diagnostic applications.
