Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Coaching-Based Training for Underrepresented Mentors in STEM.

Education sciences·2025

Same author

Creating an Integrated Coaching Culture in Higher Education.

Journal of appreciative education·2024

Same author

Strategy for Conjugating Oligopeptides to Mesoporous Silica Nanoparticles Using Diazirine-Based Heterobifunctional Linkers.

Nanomaterials (Basel, Switzerland)·2022

Same author

STEM Through Authentic Research and Training Program (START) for Underrepresented Communities: Adapting to the COVID-19 Pandemic.

Journal of STEM outreach·2022

Same author

GDNF clinical trials for Parkinson's disease: a critical human dimension.

Cell and tissue research·2020

Same author

GDNF revisited: A novel mammalian cell-derived variant form of GDNF increases dopamine turnover and improves brain biodistribution.

Neuropharmacology·2018

Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 24, 2026

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

High-quality combinatorial protein libraries using the binary patterning approach.

Luke H Bradley¹

¹Departments of Anatomy and Neurobiology, Molecular and Cellular Biochemistry, and the Center of Structural Biology, University of Kentucky College of Medicine, MN225 Chandler Medical Center, 800 Rose Street, Lexington, KY, 40536-0298, USA, lhbradley@uky.edu.

Methods in Molecular Biology (Clifton, N.J.)

|September 13, 2014

Summary

This summary is machine-generated.

Designing protein libraries with specific structures accelerates the discovery of novel protein functions for research and medicine. The binary patterning approach balances rational design with combinatorial diversity for improved protein library quality.

More Related Videos

Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries

Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries

Published on: September 6, 2012

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

Published on: December 6, 2017

Related Experiment Videos

Last Updated: Apr 24, 2026

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries

Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries

Published on: September 6, 2012

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

Published on: December 6, 2017

Area of Science:

Protein engineering
Synthetic biology
Biotechnology

Background:

Protein combinatorial libraries are crucial for discovering novel protein functions.
High-quality libraries with folded and soluble proteins are needed for efficient screening and selection.
Existing methods may not fully optimize protein structure and solubility.

Purpose of the Study:

To present the binary patterning approach for designing protein libraries.
To outline considerations for constructing binary patterned libraries of de novo and natural proteins.
To enhance the generation of folded and soluble protein libraries for functional discovery.

Main Methods:

The binary patterning approach combines rational design with combinatorial diversity.
This method specifies the arrangement of polar and nonpolar amino acid residues.
It utilizes a desired, folded tertiary structure template.

Main Results:

The binary patterning approach facilitates the creation of protein libraries rich in folded and soluble structures.
This method aids in exploring protein sequence space for novel functions.
It provides a framework for designing libraries with improved structural integrity.

Conclusions:

The binary patterning approach is a valuable strategy for protein library design.
It enhances the efficiency of isolating proteins with desired functions.
This method supports advancements in protein engineering for various applications.