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Updated: Apr 23, 2026
Nanoparticle Tracking Analysis of Gold Nanoparticles in Aqueous Media through an Inter-Laboratory Comparison
Published on: October 20, 2020
Refractive index determination of nanoparticles in suspension using nanoparticle tracking analysis
Edwin van der Pol1, Frank A W Coumans, Auguste Sturk
1Biomedical Engineering and Physics, ‡ Laboratory of Experimental Clinical Chemistry, Academic Medical Center, University of Amsterdam , Amsterdam, The Netherlands.
Abstract:
The refractive index (RI) dictates interaction between light and nanoparticles and therefore is important to health, environmental, and materials sciences. Using nanoparticle tracking analysis, we have determined the RI of heterogeneous particles <500 nm in suspension. We demonstrate feasibility of distinguishing silica and polystyrene beads based on their RI. The hitherto unknown RI of extracellular vesicles from human urine was determined at 1.37 (mean). This method enables differentiation of single nanoparticles based on their RI.
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