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Updated: Apr 23, 2026

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
Published on: April 16, 2015
Overview of orchestration of CD4+ T cell subsets in immune responses
1State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai, 200031, China, bsun@sibs.ac.cn.
Abstract:
Adaptive immunity plays an important role in the host defense of pathogens, among which CD4+ T helper cell takes a major part. The regulation of Th cell differentiation, the function they exerts in immune response, autoimmune diseases, and allergic conditions, has long attracted much attention. Naive CD4+ T cells differentiate into distinct subsets after receiving TCR and costimulation signaling for activation and cytokine signaling to direct their differentiation. In this chapter, we will have a broad overview of all Th cell subsets, including Th1, Th2, Th17, Treg, Tfh, as well as Th9 and Th22.
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