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Updated: Apr 21, 2026

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Published on: April 19, 2019

Trypsinization-dependent cell labeling with fluorescent nanoparticles.

Tetiana Serdiuk¹, Sergei Alekseev², Vladimir Lysenko³

¹University of Lyon, CarMeN Laboratory, INSA de Lyon, UMR INSERM 1060, Lyon, France ; Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64, Volodymyrska St., 01601 Kyiv, Ukraine.

Nanoscale Research Letters

|October 21, 2014

Summary

This summary is machine-generated.

Trypsinization damages cell membranes, increasing nanoparticle uptake. Wait at least 48 hours after using trypsin before exposing cells to nanoparticle (NP) labels to prevent this effect.

Keywords:

Cell labeling Fluorescent nanoparticles Trypsin

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Area of Science:

Cell biology
Biotechnology
Nanotechnology

Background:

Trypsin is commonly used for cell detachment in subculturing.
Proteolytic activity of trypsin can damage cell membrane proteins.

Purpose of the Study:

To investigate the impact of trypsinization on cellular uptake of fluorescent nanoparticles.
To determine the optimal time interval between trypsinization and nanoparticle exposure.

Main Methods:

Cell culture and trypsinization.
Exposure of cells to fluorescent nanoparticle (NP)-based labels at various time points post-trypsinization.
Quantification of cellular nanoparticle uptake using fluorescence microscopy or flow cytometry.

Main Results:

Cellular uptake of fluorescent nanoparticles significantly increased within 24 hours after trypsinization.
Trypsin-induced protein digestion leads to a compromised, leaky cell plasma membrane.
Enhanced nanoparticle uptake is directly correlated with the degree of membrane damage.

Conclusions:

Trypsinization compromises cell membrane integrity, enhancing nanoparticle uptake.
A waiting period of at least 48 hours post-trypsinization is recommended before nanoparticle labeling.
This finding is crucial for accurate nanoparticle uptake studies and experimental design.