Depth resolved hyperspectral imaging spectrometer based on structured light illumination and Fourier transform...

Area of Science:

• Optical Imaging
• Spectroscopy
• Biophotonics

Background:

• Standard imaging spectrometers lack depth resolution.
• Hyperspectral imaging provides spectral information but often in 2D.
• Depth-resolved spectral data is crucial for analyzing complex biological structures.

Purpose of the Study:

• To develop a hyperspectral imaging spectrometer with depth resolution.
• To enable 3D spectral analysis of biological specimens.
• To validate the system's performance using in vitro and biological samples.

Main Methods:

• Utilized a Sagnac interferometer for spectral cube acquisition.
• Employed structured illumination and the HiLo algorithm for depth recovery.
• Post-processed spectral data (x, y, λ) to achieve depth resolution.
• Validated with fluorescent solutions, beads, and biological tissues.

Main Results:

• Achieved depth resolution of 1.8 μm.
• Demonstrated spectral resolution of 7 nm.
• Successfully quantified spectra from 3D resolved features in biological specimens.
• Validated the system's capability for depth-resolved hyperspectral imaging.

Conclusions:

• The developed hyperspectral imaging spectrometer effectively provides depth-resolved spectral information.
• The system enables precise spectral analysis of 3D biological structures.
• This technique advances optical imaging for biological and spectral applications.