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[Changes in the functional activity of polymorphonuclear leukocytes in pseudotubercular infection]
Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
|January 1, 1989
Summary
Yersinia pseudotuberculosis infection in rabbits stimulates polymorphonuclear leukocytes (PNL) function, indicated by increased myeloperoxidase activity and cationic proteins. This immune response may contribute to impaired bacterial digestion by leukocytes.
Area of Science:
- Immunology
- Microbiology
- Cell Biology
Context:
- Investigates the immune response in rabbits following oral infection with Yersinia pseudotuberculosis.
- Focuses on the functional activity of peripheral blood polymorphonuclear leukocytes (PNL).
Purpose:
- To evaluate the changes in PNL myeloperoxidase system activity and cationic protein content during Y. pseudotuberculosis infection.
- To understand the impact of infection on leukocyte function and bacterial digestion.
Summary:
- Oral Y. pseudotuberculosis infection in rabbits led to significant stimulation of the PNL myeloperoxidase system, evidenced by a 3.32-fold increase in the nitro blue tetrazolium (NBT)-positive leukocyte index by day 5.
- A marked increase in nonenzymatic cationic PNL proteins was observed, with the median cytochemical coefficient reaching 2.035 +/- 0.005 units by day 12, compared to 0.995 +/- 0.002 in controls.
- A secondary, less pronounced increase in NBT-positive PNL and cationic proteins occurred by day 20, suggesting a sustained but diminished immune response.
Impact:
- Findings indicate faintly pronounced secretory degranulation of PNL, potentially explaining impaired digestion of Y. pseudotuberculosis by leukocytes despite retained phagocytic capacity.
- Provides insights into leukocyte dysfunction during bacterial infections and its implications for host defense mechanisms.