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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Apr 19, 2026

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Micro, soft, windows: integrating super-resolution viewing capabilities into soft lithographic devices.

Christopher Moraes¹

¹Department of Chemical Engineering, McGill University, Montreal, QC, Canada. chris.moraes@mcgill.ca.

Integrative Biology : Quantitative Biosciences From Nano to Macro

|December 17, 2014

Summary

This summary is machine-generated.

Microfluidic cell culture platforms enable precise cell studies. Innovations in these systems, combined with advanced microscopy, allow high-resolution optical probing of cellular processes.

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Last Updated: Apr 19, 2026

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Published on: November 16, 2019

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Area of Science:

Biotechnology
Cell Biology
Microfluidics

Background:

Microengineered cell culture provides controlled, physiologically relevant environments.
Optical probing of cellular processes is crucial for understanding biological mechanisms.
Super-resolution imaging and light sheet microscopy have advanced biological observation.

Purpose of the Study:

To survey recent innovations in microfluidic cell culture platforms.
To highlight how these platforms enable high-spatial and temporal-resolution probing of biological activity.
To connect these advances with breakthroughs in advanced microscopy techniques.

Main Methods:

Review of recent innovations in microfluidic cell culture platform design.
Discussion of the integration of microfluidics with advanced optical microscopy.
Focus on technologies enabling high-resolution imaging of cellular processes.

Main Results:

Emerging microfluidic platforms offer enhanced control over cellular environments.
Integration with super-resolution and light sheet microscopy allows unprecedented visualization.
These combined technologies facilitate detailed study of cellular and sub-cellular activities.

Conclusions:

Microfluidic cell culture systems are advancing the study of biological processes.
High-resolution optical probing within these platforms provides new insights.
Technological integration offers novel windows into cellular mechanisms.