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A New Straightforward Method for Lipophilicity logP Measurement using 19F NMR Spectroscopy
Published on: January 30, 2019
Liposcale: a novel advanced lipoprotein test based on 2D diffusion-ordered 1H NMR spectroscopy
Roger Mallol1, Núria Amigó1, Miguel A Rodríguez2
1Department of Electronic Engineering, Universitat Rovira i Virgili, IISPV, Tarragona, Spain; Spanish Biomedical Research Centre in Diabetes and Associated Metabolic Disorders (CIBERDEM), Madrid, Spain; Biosfer Teslab, Reus, Spain.
The novel Liposcale test, using advanced NMR, accurately measures lipoprotein particle size and number for improved cardiovascular risk prediction. It shows stronger correlations and better concordance than existing tests, aiding in identifying conditions like atherogenic dyslipidemia.
Area of Science:
- Biochemistry
- Cardiovascular Medicine
- Analytical Chemistry
Background:
- Accurate measurement of lipoprotein particle size and number is crucial for predicting cardiovascular disease risk.
- Advanced Lipoprotein Tests (ALTs) offer improved insights beyond traditional lipid panels.
- Existing ALTs have limitations in precision and correlation with clinical markers.
Purpose of the Study:
- To introduce and validate the Liposcale test, a novel ALT utilizing 2D diffusion-ordered (1)H NMR spectroscopy.
- To assess the Liposcale test's performance in measuring lipoprotein particle size and number.
- To compare the Liposcale test's accuracy and reliability against established methods and clinical conditions.
Main Methods:
- Development of the Liposcale test based on 2D diffusion-ordered (1)H NMR spectroscopy.
- Utilizing diffusion coefficients for direct measurement of mean lipoprotein particle sizes and numbers.
- Validation using 177 plasma samples from healthy individuals and 307 type 2 diabetic patients.
Main Results:
- The Liposcale test demonstrated stronger correlations between NMR-derived lipoprotein particle numbers and apolipoprotein concentrations compared to the LipoProfile test.
- LDL particle number conversions to ApoB equivalents showed comparable results to the LipoProfile test.
- HDL particle number measurements exhibited higher concordance with ion mobility-determined values.
- Principal component analysis using Liposcale data effectively distinguished type 2 diabetic patients with and without atherogenic dyslipidemia (AUC = 0.88).
Conclusions:
- The Liposcale test provides a reproducible and reliable method for characterizing lipoprotein particles.
- This novel NMR-based approach offers significant advantages for cardiovascular risk assessment.
- The Liposcale test is applicable to pathological states, including atherogenic dyslipidemia in type 2 diabetes.
