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A gastrointestinal transit study on amphotericin B-loaded solid lipid nanoparticles in rats
Hilda Amekyeh1, Nashiru Billa, Kah-Hay Yuen
1School of Pharmacy, University of Nottingham, Malaysia Campus, 43500, Semenyih, Selangor, Malaysia.
AAPS Pharmscitech
|January 16, 2015
Summary
This study investigated amphotericin B (AmB) solid lipid nanoformulation (SLN) gastrointestinal transit in rats. AmB SLN showed favorable absorption from the small intestine, providing insights into its absorption window.
Area of Science:
- Pharmacokinetics
- Drug Delivery Systems
- Nanotechnology
Background:
- Gastrointestinal (GI) transit influences drug absorption.
- Solid lipid nanoformulations (SLNs) offer potential for drug delivery.
- Understanding amphotericin B (AmB) SLN behavior is crucial for optimizing its therapeutic use.
Purpose of the Study:
- To investigate the GI transit behavior of AmB SLN in rats.
- To estimate gastric emptying time (GET) and cecal arrival time (CAT) of AmB SLN.
- To determine the absorption window of AmB from SLN.
Main Methods:
- Formulation and characterization of AmB, paracetamol (PAR), and sulfasalazine (SSZ) SLNs.
- Indirect estimation of GET and CAT using marker drugs PAR and sulfapyridine (SP).
- Assessment of in vitro drug release, morphology, and in vivo absorption.
Main Results:
- All SLNs exhibited similar physicochemical properties.
- PAR demonstrated rapid small intestine absorption (T50E = 1.6 h).
- SP indicated SLN arrival in the colon after 2 h, serving as CAT; AmB was absorbed slowly but favorably from the small intestine.
Conclusions:
- AmB SLN demonstrates favorable absorption characteristics within the small intestine.
- The study provides valuable data on AmB SLN GI transit and absorption window.
- This research supports the potential of AmB SLN for improved drug delivery.
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