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[Cryopreservation of hemopoietic stem cells in autologous bone marrow transplantation]
Autologous bone-marrow transplantation (AKMT) is employed to shorten the period of aplasia following aggressive radiotherapy and chemotherapy. Deep freezing of haemopoietic stem cells (HS) must be undertaken in this connection. In order to achieve the greatest survival of HS, the following requirements must be fulfilled: 1) The use of acid citrate dextrose as an anticoagulation agent is recommended. 2) The best results are obtained if a concentrate of mononuclear cells is deep frozen. 3) A cryoprotective agent must be employed in order to avoid injurious osmotic gradients through the cell membrane. Ten percent di-methyl sulphoxide has been most tested and is most suitable. Cell concentrations before freezing must be 1-5 x 10(7) nuclear cells (NBC) ml. 4) Cells must be frozen in a homogenous 3-4 mm thick layer in special bags made of temperature-resistant material. 5) Controlled freezing at a rate of between 1 and 2 degrees C/minute is employed. The heat yield at the eutechnical point must be eliminated. 6) Storage must be undertaken at temperatures lower than -140 degrees C. Bone-marrow should not be employed after storage for three years. 7) Agar culture is employed as a screening test to demonstrate if an adequate number of cells are available. 8) The minimum dosage at AKMT is assessed as 1-7 x 10(7) NBC/kg. 9) The threshold dosage at AKMT is assessed to be 5-10 x 10(7) NBC/kg. 10) Thawing must be done rapidly. 11) The infusion should be concluded at the latest 15 minutes after thawing.(ABSTRACT TRUNCATED AT 250 WORDS)
Autologous bone-marrow transplantation (AKMT) is employed to shorten the period of aplasia following aggressive radiotherapy and chemotherapy. Deep freezing of haemopoietic stem cells (HS) must be undertaken in this connection. In order to achieve the greatest survival of HS, the following requirements must be fulfilled: 1) The use of acid citrate dextrose as an anticoagulation agent is recommended. 2) The best results are obtained if a concentrate of mononuclear cells is deep frozen. 3) A cryoprotective agent must be employed in order to avoid injurious osmotic gradients through the cell membrane. Ten percent di-methyl sulphoxide has been most tested and is most suitable. Cell concentrations before freezing must be 1-5 x 10(7) nuclear cells (NBC) ml. 4) Cells must be frozen in a homogenous 3-4 mm thick layer in special bags made of temperature-resistant material. 5) Controlled freezing at a rate of between 1 and 2 degrees C/minute is employed. The heat yield at the eutechnical point must be eliminated. 6) Storage must be undertaken at temperatures lower than -140 degrees C. Bone-marrow should not be employed after storage for three years. 7) Agar culture is employed as a screening test to demonstrate if an adequate number of cells are available. 8) The minimum dosage at AKMT is assessed as 1-7 x 10(7) NBC/kg. 9) The threshold dosage at AKMT is assessed to be 5-10 x 10(7) NBC/kg. 10) Thawing must be done rapidly. 11) The infusion should be concluded at the latest 15 minutes after thawing.(ABSTRACT TRUNCATED AT 250 WORDS)