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Upregulation of miR-362-3p Modulates Proliferation and Anchorage-Independent Growth by Directly Targeting Tob2 in

Hongxing Shen1, Wenjuan Li1, Yuan Tian1

  • 1College of Life Sciences, Wuhan University, Wuhan 430072, China.

Journal of Cellular Biochemistry
|February 5, 2015
PubMed

Insights

Dysregulation of microRNA-362-3p (miR-362-3p) and Tob2 contributes to hepatocellular carcinoma (HCC) development. Epigenetic changes affect miR-362-3p, impacting HCC cell growth and malignancy.

Area of Science:

  • Molecular Biology
  • Oncology
  • Epigenetics

Background:

  • MicroRNA (miRNA) dysregulation is implicated in human cancer pathogenesis.
  • Epigenetic modifications, including DNA methylation, can alter miRNA expression.
  • Hepatocellular carcinoma (HCC) is a major malignancy where miRNA roles are increasingly recognized.

Purpose of the Study:

  • To identify microRNAs regulated by DNA methylation in hepatocellular carcinoma.
  • To investigate the role of miR-362-3p and its target Tob2 in HCC development.

Main Methods:

  • Global miRNA expression profiling of HCC and normal cell lines treated with DNA methylation and histone deacetylase inhibitors (5-Aza-2'-deoxycytidine and Trichostatin A).
  • Analysis of miR-362-3p promoter methylation and expression in HCC cells and tissues.
  • Functional studies involving ectopic expression and inhibition of miR-362-3p and Tob2 in HCC cells.

Main Results:

  • Epigenetic drugs differentially modulated miRNA expression in HCC cells.
  • miR-362-3p expression increased, while its promoter methylation decreased in HCC cells and tissues.
  • miR-362-3p overexpression promoted HCC cell proliferation and anchorage-independent growth by targeting Tob2.
  • Tob2 expression was reduced in HCC tissues, and its regulation by miR-362-3p was confirmed.

Conclusions:

  • Dysregulation of miR-362-3p and its target Tob2 is associated with hepatocellular carcinoma malignancy.
  • Epigenetic mechanisms, particularly DNA methylation, play a role in regulating miR-362-3p in HCC.
  • miR-362-3p and Tob2 represent potential therapeutic targets for HCC.
Keywords:
EPIGENETIC REGULATIONHEPATOCELLULAR CARCINOMATob2microRNA-362-3p

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