Related Experiment Video
Updated: Apr 17, 2026
Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay
Published on: January 8, 2012
Isolation and culturing of trophoblasts from human terminal placenta
T D Kolokol'tsova1, N N Saburina, P A Poltavtseva
1Research Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, Russia, kolokoltd@mail.ru.
Abstract:
Trophoblast culture was derived from the islets of cell migrating from the fragments of placental microvilli. The isolated cells retained their cytotrophoblast phenotype longer (until passage 2-3) and then gained characteristics of proliferating mesenchymal cells. We conclude that changes in the morphology and co-expression of epithelial (cytokeratin 7) and mesenchymal (vimentin) markers attest to epithelial mesenchymal transition.
