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Related Concept Videos

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling02:24

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
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Related Experiment Video

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Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses
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Whole transcriptome expression profiling of mouse limb tendon development by using RNA-seq.

Han Liu1, Jingyue Xu, Chia-Feng Liu

  • 1Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, MLC 7007, Cincinnati, Ohio, 45229.

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
|March 3, 2015
PubMed
Summary
This summary is machine-generated.

This study identifies key genes involved in tendon development using Scx-GFP mice. These findings offer insights into the molecular mechanisms regulating tendon formation and differentiation.

Keywords:
Foxf2RNA-seqScxSix2tendon

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Tendon development mechanisms are poorly understood compared to bone and muscle.
  • Fibrous connective tissues like tendons are crucial for force transmission.

Purpose of the Study:

  • To identify genes and molecular pathways regulating tendon development.
  • To characterize early gene expression in tendon progenitor cells.

Main Methods:

  • Whole transcriptome RNA-sequencing of Scx-GFP positive and negative cells from developing mouse limbs.
  • Analysis of gene expression changes at different developmental stages (E11.5, E13.5, E15.5).
  • In situ hybridization to validate gene expression patterns.

Main Results:

  • Over 1,500 genes showed enriched expression in GFP(+) tendon progenitor cells.
  • Identified temporal gene expression patterns, with significant upregulation from E11.5 to E15.5.
  • Discovered over 30 transcription factor genes activated early in tendon development.
  • Foxf2 identified as a specific marker for differentiated dorsal limb tendon cells.

Conclusions:

  • Provides a comprehensive gene expression dataset for tendon development.
  • Highlights the role of specific transcription factors in tendon progenitor cell activation.
  • Foxf2 is a potential marker for mature tendon cells, aiding future research.