Near infrared lasers in flow cytometry

Area of Science:

• Biotechnology
• Analytical Chemistry
• Optical Engineering

Background:

• Flow cytometry technology has historically relied on laser advancements for fluorescent probe excitation.
• Early flow cytometers used limited, large lasers, while modern systems leverage solid-state lasers for broader wavelength capabilities.

Purpose of the Study:

• To review available technology for near-infrared (NIR) flow cytometry.
• To explore the potential of the NIR spectrum to expand the number of simultaneously analyzed fluorescent probes.

Main Methods:

• Review of current laser technologies (UV to NIR), including solid-state and diode lasers.
• Discussion of fluorescent probe development for various spectral regions.
• Examination of detector technologies suitable for NIR signal detection.

Main Results:

• Modern flow cytometers can simultaneously analyze over 20 fluorescent probes using multiple laser wavelengths.
• The near-infrared spectrum (660-800 nm) remains largely unexplored in flow cytometry despite available laser technology.
• NIR fluorescent probes used in imaging and tracking are potential candidates for flow cytometry applications.

Conclusions:

• The near-infrared region represents a significant frontier for increasing multiplexing capabilities in flow cytometry.
• Incorporating NIR laser diodes and appropriate fluorescent probes can accelerate advancements in cellular analysis.
• Further development in NIR fluorescent probes and detection systems is crucial for maximizing flow cytometry's potential.