Related Experiment Video
Updated: Apr 15, 2026
15:03
Synthesis of Functionalized Magnetic Nanoparticles, Their Conjugation with the Siderophore Feroxamine and its Evaluation for Bacteria Detection
Published on: June 16, 2020
10.1K
Biocompatible capped iron oxide nanoparticles for Vibrio cholerae detection.
Anshu Sharma1, Dinesh Baral, Kamla Rawat
1Special Centre for Nanosciences, Jawaharlal Nehru University, New Delhi-110067, India. School of Physical Science, Jawaharlal Nehru University, New Delhi-110067, India.
Nanotechnology
|April 9, 2015
Summary
This study developed an electrochemical immunosensor for detecting Vibrio cholerae using magnetite nanoparticles. The novel sensor demonstrates high sensitivity and reproducibility for rapid pathogen identification.
Area of Science:
- Biosensors and Nanotechnology
- Electrochemical Sensing
- Immunodiagnostics
Background:
- Vibrio cholerae poses a significant public health threat, necessitating rapid and sensitive detection methods.
- Current diagnostic techniques can be time-consuming and require specialized laboratory equipment.
- Nanoparticle-based immunosensors offer a promising platform for enhanced sensitivity and specificity in pathogen detection.
Purpose of the Study:
- To fabricate and characterize an efficient electrochemical immunosensor for Vibrio cholerae detection.
- To utilize functionalized magnetite nanoparticles for improved signal amplification and immobilization.
- To evaluate the analytical performance of the developed immunosensor.
Main Methods:
- Synthesis of citric acid-capped magnetite (Fe3O4) nanoparticles via co-precipitation.
- Electrophoretic deposition of nanoparticles onto an indium-tin-oxide (ITO) coated glass substrate.
- Immobilization of monoclonal antibodies against Vibrio cholerae and bovine serum albumin (BSA) for immunosensor construction.
- Characterization using XRD, TEM, FTIR, and DLS.
- Electrochemical measurements to assess sensor performance.
Main Results:
- Fe3O4 and CA-Fe3O4 nanoparticles were successfully synthesized and characterized, with average crystalline sizes of 29 ± 1 nm and 37 ± 1 nm, respectively.
- Dynamic Light Scattering (DLS) measurements indicated hydrodynamic radii of 77.35 nm for Fe3O4 and 189.51 nm for CA-Fe3O4.
- The fabricated immunosensor demonstrated a wide detection range (12.5–500 ng mL−1), a low detection limit (0.32 ng mL−1), and high sensitivity (0.03 Ω/ng ml−1 cm−2).
- Excellent reproducibility was observed, with the sensor functioning effectively for over 11 detection cycles.
Conclusions:
- The developed electrochemical immunosensor based on citric acid-functionalized magnetite nanoparticles is highly efficient for Vibrio cholerae detection.
- The sensor exhibits excellent sensitivity, a broad detection range, and robust reproducibility, making it suitable for practical diagnostic applications.
- This approach represents a significant advancement in the development of rapid and reliable tools for cholera surveillance and control.
