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Steroid profile for urine: reference values.

C W Weykamp1, T J Penders, N A Schmidt

  • 1Streekziekenhuis Koningin Beatrix, Winterswijk, The Netherlands.

Clinical Chemistry
|December 1, 1989
PubMed
Summary
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This study established normal urine steroid reference values for men and women across six age groups using capillary gas chromatography. These reliable values aid in the quantitative interpretation of steroid profiles.

Area of Science:

  • Endocrinology
  • Clinical Chemistry
  • Analytical Chemistry

Background:

  • Accurate steroid profiling requires established normal reference values.
  • Previous reference values may lack comprehensive data across diverse populations and age groups.

Purpose of the Study:

  • To determine reliable, population-based reference values for urinary steroids in healthy individuals.
  • To establish reference ranges for specific steroids, ratios, and discriminant functions for conditions like polycystic ovarian disease.
  • To provide a foundation for the quantitative interpretation of steroid profiles in clinical diagnostics.

Main Methods:

  • Capillary gas chromatography was employed for the analysis of urine samples.
  • Urine from 288 healthy volunteers across The Netherlands and Belgium was analyzed in triplicate.

Related Experiment Videos

  • Reference values were determined for a comprehensive panel of steroids, ratios, and urinary parameters.

    • Main Results:

    • Normal reference values (in mumol/24 h) were established for 15 different steroids, including androsterone, etiocholanolone, and various cortisol metabolites.
    • Key reference ratios (e.g., etiocholanolone/androsterone) and a discriminant function for polycystic ovarian disease were determined.
    • Reference values were stratified by sex and six distinct age categories, from infants to the elderly.

    Conclusions:

    • The determined reference values are reliable and suitable for the quantitative interpretation of steroid profiles.
    • This study provides a robust dataset for clinical laboratories and researchers in endocrinology and related fields.
    • The established reference ranges enhance diagnostic accuracy for various endocrine conditions.