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Characterizing Cell Migration Within Three-dimensional In Vitro Wound Environments
Published on: August 16, 2017
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Coagulation factor IX regulates cell migration and adhesion in vitro.
Hisataka Kitano1, Atsushi Mamiya1, Ishikawa Tomomi2
1Division of Dental Surgery, Nihon University School of Medicine, Tokyo, Japan.
Cell Biology International
|May 16, 2015
Summary
Coagulation factor IX (FIX) has distinct cellular effects depending on its activation state. Intact FIX suppresses cell migration, while activated FIX promotes it, revealing new roles for FIX in cellular processes.
Area of Science:
- Biochemistry
- Cell Biology
- Hematology
Background:
- Coagulation factor IX (FIX) circulates as an inactive zymogen.
- The cellular functions of FIX are not well understood.
Purpose of the Study:
- To investigate the effects of intact and activated FIX on cell behavior.
- To identify the specific domains responsible for FIX activity.
Main Methods:
- Utilized A431, Pro5, Cos7, and human umbilical vein endothelial cells.
- Performed wound-healing and modified Boyden chamber assays.
- Analyzed the impact of FIX processing on cell migration and adhesion.
Main Results:
- Full-length FIX suppressed cell migration and increased adhesion.
- Activated FIX enhanced cell migration and reduced adhesion.
- The activation peptide and an EGF domain were identified as key active regions.
Conclusions:
- FIX processing significantly alters its cellular effects.
- FIX plays roles in cellular processes beyond coagulation.
- Distinct FIX forms exhibit opposing effects on cell migration and adhesion.
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