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Updated: Apr 12, 2026

Anionic Polymerization of an Amphiphilic Copolymer for Preparation of Block Copolymer Micelles Stabilized by π-π Stacking Interactions
Published on: October 10, 2016
A general method for synthesizing enzyme-polymer conjugates in reverse emulsions using Pluronic as a reactive
Xiaoling Wu1, Jun Ge, Jingying Zhu
1Key Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China. junge@mail.tsinghua.edu.cn.
Abstract:
Using aldehyde-functionalized Pluronic as the reactive surfactant, enzyme-Pluronic conjugates with sizes ranging from nanometers to micrometers were synthesized in reverse emulsions. Compared with the direct conjugation in aqueous solution, this method gave an increased conjugation efficiency and well-controlled size of the conjugates. The versatility of this method was validated using horseradish peroxidase (HRP), Candida rugosa lipase (CRL) and Candida antarctica lipase B (CALB). The resulting enzyme-Pluronic conjugates showed greatly enhanced apparent activity compared to free enzymes in organic media.
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