Polyphenols in Ammania auriculata: structures, antioxidative activity and cytotoxicity

Area of Science:

• Phytochemistry
• Pharmacology
• Natural Products Chemistry

Background:

• Ammania auriculata is a plant with potential medicinal properties.
• Polyphenols are known for their antioxidant and potential therapeutic effects.
• Understanding the chemical constituents and bioactivity of medicinal plants is crucial.

Purpose of the Study:

• To isolate and identify polyphenols from Ammania auriculata.
• To evaluate the antioxidant and cytotoxic activities of the isolated compounds.
• To characterize a novel galloyl glucose derivative.

Main Methods:

• Phytochemical investigation using spectroscopic methods (1D and 2D NMR, HR-ESIMS).
• Antioxidant activity assessment using DPPH and ORAC assays.
• Cytotoxicity evaluation on HaCaT cells via neutral red uptake and cell cycle analysis.

Main Results:

• Eight polyphenols were identified, including a novel galloyl glucose (8).
• Compounds 1, 3, 4, 5, and 6 demonstrated significant antioxidant activity (ED50: 3.22–9.79 μg/ml).
• Compounds 1, 3, 4, and 5 exhibited cytotoxic effects (IC50: 30.7–84.1 μg/ml), while compound 8 was non-cytotoxic.

Conclusions:

• Ammania auriculata is a source of bioactive polyphenols with notable antioxidant properties.
• Specific compounds possess both antioxidant and cytotoxic potential, warranting further investigation.
• The novel galloyl glucose derivative shows promise due to its lack of cytotoxicity.