Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Gradient metapopulation microfluidic ecologies shape genetic and biofilm drivers of T4r phage resistance in E. coli.

NPJ biofilms and microbiomes·2026

Same author

Informational Memory Shapes Collective Behavior in Intelligent Swarms.

Physical review letters·2026

Same author

Liposome purification from micromolar protein background using diffusiophoretic trapping.

Nanoscale·2026

Same author

Tunable single-column deterministic lateral displacement device by adjustable crossflow.

Lab on a chip·2026

Same author

Protocol to model tumor hypoxia in vitro using real-time phosphorescence-based sensing of O<sub>2</sub> gradients generated by metastatic cancer cells.

STAR protocols·2025

Same author

Calibrating photomultiplier tubes beyond their performance envelope.

The Review of scientific instruments·2025

Search research articles

Related Experiment Video

Updated: Apr 11, 2026

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

Concentrating genomic length DNA in a microfabricated array.

Yu Chen^1,2, Ezra S Abrams³, T Christian Boles³

¹Princeton Institute for Science and Technology of Materials (PRISM), Princeton, New Jersey 08540, USA.

Physical Review Letters

|May 30, 2015

Summary

This summary is machine-generated.

We show that microfabricated bump arrays can concentrate long DNA molecules at high flow speeds by compacting them with polyethylene glycol (PEG). This method is crucial for purifying DNA for next-generation sequencing libraries.

More Related Videos

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

High-throughput Protein Expression Generator Using a Microfluidic Platform

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

Related Experiment Videos

Last Updated: Apr 11, 2026

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

High-throughput Protein Expression Generator Using a Microfluidic Platform

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

Area of Science:

Biotechnology
Materials Science
Molecular Biology

Background:

Genomic-length DNA molecules are challenging to concentrate at high flow rates.
Efficient DNA concentration is critical for applications like next-generation sequencing library preparation.

Purpose of the Study:

To demonstrate a microfluidic method for concentrating genomic-length DNA molecules.
To identify optimal conditions for DNA concentration using microfabricated bump arrays and polyethylene glycol (PEG).

Main Methods:

Utilized microfabricated bump arrays for DNA molecule manipulation.
Employed polyethylene glycol (PEG) depletion forces to compact DNA molecules and increase their shear modulus.
Investigated the effects of PEG concentration and flow speed on DNA concentration efficiency through theoretical analysis and experiments.

Main Results:

Achieved efficient concentration of genomic-length DNA molecules at continuous, high flow velocities (up to 40 μm/s).
Demonstrated that DNA molecule compaction via PEG is essential for achieving concentration at high flow rates.
Mapped the optimal operating window (sweet spot) for DNA concentration as a function of PEG concentration and flow speed.

Conclusions:

Microfabricated bump arrays offer an efficient method for concentrating genomic-length DNA.
PEG-mediated compaction is key to enabling high-throughput DNA concentration in microfluidic devices.
This technique has significant potential for purifying DNA in next-generation sequencing library preparation workflows.