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Published on: April 12, 2018
Optimization of the Method of Buccal Epithelial Cell Isolation and Culturing on Collagen Substrate for Ophthalmologic
E V Chentsova1, O I Konyushko, M S Makarov
1Helmholtz Moscow Research Institute of Eye Diseases, Ministry of Health of the Russian Federation, Moscow, Russia.
Abstract:
We performed morphofunctional analysis of cultured rabbit buccal epithelial cells in the primary culture, during passaging, and upon interaction with collagen substrates. Three different morphological types were identified. It was demonstrated that precursors of rabbit buccal epithelium were characterized by high proliferative activity, retain their biological properties throughout long-term culturing, and adhere to various collagen substrates.
