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Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
Published on: August 2, 2018
Expression of HIF-1α in Multipotent Mesenchymal Stromal Cells under Hypoxic Conditions
1State Scientific Center Institute of Biomedical Problems, Russian Academy of Medical Sciences, Moscow, Russia, pogodina_m@mail.ru.
Abstract:
According to the data of reverse-transcription-PCR, long-term culturing of multipotent mesenchymal stromal cells from human adipose tissue at 1% O2 (vs. standard 20% O2) led to a decrease in HIF-1α gene expression, while no changes in this parameter were revealed at 5% O2. Analysis of the time course of HIF-1α mRNA level showed that at the early terms of hypoxic exposure, the expression of this gene increased and the duration of this increase depended on the severity of hypoxia.
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