Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Experiences of restrictive practices in child and adolescent mental health inpatient care: an integrative review.

Child and adolescent psychiatry and mental health·2026
Same author

1390 nm GaAs-based dilute nitride VCSELs on 150 mm wafers for scalable production.

Optics express·2026
Same author

Topographical memory in transient epileptic amnesia.

Epilepsy & behavior : E&B·2026
Same author

Co-production as the ultimate goal; an incentive or discouragement?

Research involvement and engagement·2025
Same author

Evidence of Genetic Isolation and Differentiation Among Historically Fragmented British Populations of Common Juniper, <i>Juniperus communis</i> L.

Ecology and evolution·2025
Same author

Author Correction: Electrical stimulation of smiling muscles reduces visual processing load and enhances happiness perception in neutral faces.

Communications psychology·2025

Related Experiment Video

Updated: Apr 6, 2026

Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts
13:16

Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts

Published on: December 22, 2015

12.0K

Validation of Histologic Bone Analysis Following Microfil Vessel Perfusion.

D Sarhaddi1, B Poushanchi1, M Merati1

  • 1The University of Michigan: Craniofacial Anomalies Program, Pediatric Plastic Surgery, 1500 E. Medical Center Drive, F7894 Mott Children's Hospital, Ann Arbor, Mich. 48109-5219.

Journal of Histotechnology
|July 25, 2015
PubMed
Summary

Microfil® perfusion enables simultaneous examination of bone vascularity and cellularity. This study validates that Microfil® perfusion does not hinder accurate histologic assessment of bone quality, including osteocyte and empty lacunae counts.

Keywords:
CellularityHistologyMandibleMicro-Computed TomographyMicrofilPerfusionVascularity

More Related Videos

Author Spotlight: Creating Human Vascularized Micro-Tumors as Models for Translational Cancer Research
07:26

Author Spotlight: Creating Human Vascularized Micro-Tumors as Models for Translational Cancer Research

Published on: September 15, 2023

2.6K
Establishing In Situ Closed Circuit Perfusion of Lower Abdominal Organs and Hind Limbs in Mice
05:27

Establishing In Situ Closed Circuit Perfusion of Lower Abdominal Organs and Hind Limbs in Mice

Published on: August 13, 2020

6.8K

Related Experiment Videos

Last Updated: Apr 6, 2026

Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts
13:16

Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts

Published on: December 22, 2015

12.0K
Author Spotlight: Creating Human Vascularized Micro-Tumors as Models for Translational Cancer Research
07:26

Author Spotlight: Creating Human Vascularized Micro-Tumors as Models for Translational Cancer Research

Published on: September 15, 2023

2.6K
Establishing In Situ Closed Circuit Perfusion of Lower Abdominal Organs and Hind Limbs in Mice
05:27

Establishing In Situ Closed Circuit Perfusion of Lower Abdominal Organs and Hind Limbs in Mice

Published on: August 13, 2020

6.8K

Area of Science:

  • Biomedical Engineering
  • Skeletal Biology
  • Histology

Background:

  • Simultaneous analysis of bone vascularity and cellularity is crucial for understanding bone health.
  • Micro-Computed Tomography (μCT) and histology are key techniques for bone analysis.
  • Microfil® perfusion is used to visualize vasculature, but its impact on subsequent histology is unclear.

Purpose of the Study:

  • To determine if Microfil® perfusion affects the accuracy of histologic bone quality assessment.
  • To validate the combined use of Microfil® perfusion, μCT, and histology for comprehensive bone analysis.

Main Methods:

  • Sprague-Dawley rats underwent Microfil® perfusion.
  • Hemi-mandibles were analyzed using μCT for vascularity.
  • Specimens were decalcified, sectioned, stained (Gomori's Trichrome), and underwent quantitative histomorphometric evaluation.
  • Osteocyte and empty lacunae counts were compared between perfused and control groups.

Main Results:

  • Histologic analyses were successfully performed on Microfil®-perfused specimens.
  • No statistically significant difference was found in osteocyte counts between perfused and control groups (95.33 vs. 94.66).
  • No statistically significant difference was observed in empty lacunae counts between perfused and control groups (2.73 vs. 1.89).

Conclusions:

  • Microfil® perfusion does not preclude accurate histologic assessment of bone cellularity.
  • This validated method allows for simultaneous examination of bone vascular and cellular environments.
  • The combined approach enhances efficiency in studying bone structure and pathology.