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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Updated: Apr 6, 2026

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Published on: November 30, 2021

Extraction of DNA from Human Remains.

J E Stray¹, J G Shewale¹

¹Life Technologies Corporation, Foster City, CA, USA.

Forensic Science Review

|August 6, 2015

Summary

Advances in analytical methods enable genetic analysis from small bone and tooth samples. DNA recovery from ancient calcified tissues is now practical for genotypic insights.

Area of Science:

Forensic Science
Molecular Biology
Anthropology

Background:

Analytical methods have advanced, allowing genetic profiling from minimal sample quantities.
Improved DNA recovery protocols now target ancient or compromised calcified tissues like bone and teeth.

Purpose of the Study:

To review current best practices for DNA isolation and purification from bone and teeth.
To highlight lysis and purification processes for challenging skeletal samples.

Main Methods:

Focus on DNA isolation and purification techniques for bone and teeth.
Discusses lysis and purification strategies for ancient or degraded samples.

Main Results:

Highly discriminative genotypic information can be obtained from small bone and tooth samples.

Keywords:

Ancient DNA DNA extraction DNA isolation STR profiling human identification human remains missing persons mitochondrial DNA sequencing

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Last Updated: Apr 6, 2026

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

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Published on: November 30, 2021

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

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Mitochondrial and genomic DNA from skeletal remains, even ancient ones, are amenable to genotypic analysis.

Conclusions:

Modern techniques facilitate DNA recovery and genotypic analysis from challenging bone and tooth samples.
The practicality of genotyping ancient skeletal remains has significantly increased.