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Copper complexes stimulate hemopoiesis and lymphopoiesis
L S Soderberg1, J B Barnett, J R Sorenson
1Department of Microbiology and Immunology, College of Medicine, University of Arkansas for Medical Sciences, Little Rock 72205.
Advances in Experimental Medicine and Biology
|January 1, 1989
Summary
The copper complex Cu(II)2(3,5-DIPS)4 caused significant spleen enlargement and increased myelopoiesis in mice. This compound also stimulated lymphopoiesis, aiding immune recovery after irradiation.
Area of Science:
- Immunology
- Toxicology
- Biochemistry
Background:
- Copper(II)2(3,5-diisopropylsalicylate)4 [Cu(II)2(3,5-DIPS)4] is a synthetic copper complex with known radiation recovery, anti-inflammatory, and wound healing properties.
- The systemic effects of Cu(II)2(3,5-DIPS)4 on hematopoietic and immune systems require further elucidation.
Purpose of the Study:
- To investigate the effects of Cu(II)2(3,5-DIPS)4 on mouse spleen and bone marrow cellularity and myelopoiesis.
- To assess the impact of Cu(II)2(3,5-DIPS)4 on immune cell responses and recovery following irradiation.
- To determine copper distribution in spleen and bone marrow after treatment.
Main Methods:
- C57BL/6 mice were administered 80 mumol/kg of Cu(II)2(3,5-DIPS)4 subcutaneously.
- Spleen and bone marrow cellularity and myelopoiesis were analyzed.
- Mitogenic and antigenic responses of spleen cells were measured.
- Immune recovery after 8 Gy whole body irradiation was assessed.
- Copper levels in spleen and bone marrow were quantified at 24 hours and 7 days post-treatment.
Main Results:
- Subcutaneous injection of Cu(II)2(3,5-DIPS)4 led to significant spleen enlargement and increased splenic myelopoiesis lasting over 3 weeks.
- Bone marrow cellularity and myelopoiesis remained largely unchanged.
- Cu(II)2(3,5-DIPS)4 treatment minimally affected spleen cell responses to mitogenic or antigenic stimulation but stimulated lymphopoiesis.
- Immune reactivity recovery was accelerated in irradiated mice treated with Cu(II)2(3,5-DIPS)4.
- Copper levels in spleen and bone marrow were not significantly elevated 24 hours post-treatment and were transiently reduced at 7 days.
Conclusions:
- Cu(II)2(3,5-DIPS)4 induces significant splenomegaly and alters myelopoiesis in mice.
- The complex enhances lymphopoiesis and immune recovery post-irradiation, suggesting immunomodulatory effects.
- Transient changes in copper levels indicate complex pharmacokinetics and potential mechanisms of action.