Related Experiment Video
Updated: Aug 5, 2026
Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration
Published on: September 12, 2014
Cellular Response of Stem Cells on Nanofibrous Scaffold for Ocular Surface Bioengineering
Alireza Baradaran-Rafii1, Esmaeil Biazar, Saeed Heidari-Keshel
1From the *Ocular Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran; †Department of Biomaterials Engineering, Islamic Azad University, Tonekabon Branch, Iran; and ‡Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Abstract:
In this study, the human limbus stem cell was used to evaluate the phenotypic profile, viability, proliferation, and attachment ability of nanofibrous poly (3-hydroxybutyrate-co-3-hydroxyvalerate) substrates. Results indicated that the all substrates were highly biocompatible, as firmly anchored to the substrates, and were able to retain a normal corneal stem cell phenotype. Immunocytochemistry results revealed no change in the expression profile of cells grown on nanofibrous substrate when compared with those grown on amniotic membrane. In addition, nanofibrous substrate provides not only a milieu supporting cells expansion, but also serve as a useful alternative carrier for ocular surface engineering.
