Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

54
Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
54
Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

2.3K
Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at...
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bioluminescent Immunophage Sensors for the Quantification of Insulin.

ACS omega·2026
Same author

Experimental and Computational Evaluation of Nicotinamide Cofactor Biomimetics.

ACS chemical biology·2025
Same author

Selectivity in Chemiresistive Gas Sensors: Strategies and Challenges.

Chemical reviews·2025
Same author

Chiral Lemniscate Formation in Magnetic Field Controlled Topological Fluid Flows.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

A Practical Guide to 3D Printing for Chemistry and Biology Laboratories.

Current protocols·2024
Same author

Ultrafast His-Tagged Protein Purification.

Current protocols·2024
Same journal

Methods to Validate Binding and Kinetics of "Proximity-Inducing" Covalent Immune-Recruiting Molecules.

Current protocols in chemical biology·2020
Same journal

Multiparametric High-Content Assays to Measure Cell Health and Oxidative Damage as a Model for Drug-Induced Liver Injury.

Current protocols in chemical biology·2020
Same journal

Visualizing RNA Cytidine Acetyltransferase Activity by Northern Blotting.

Current protocols in chemical biology·2020
Same journal

Three-Color Imaging Enables Simultaneous Screening of Multiple RNA Targets on Small Molecule Microarrays.

Current protocols in chemical biology·2020
Same journal

Azide-Terminated RAFT Polymers for Biological Applications.

Current protocols in chemical biology·2020
Same journal

Discovery of Electrophiles and Profiling of Enzyme Cofactors.

Current protocols in chemical biology·2020
See all related articles

Related Experiment Video

Updated: Apr 4, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

12.7K

Biosensing with Virus Electrode Hybrids.

Kritika Mohan1, Reginald M Penner1,2, Gregory A Weiss1,3

  • 1Department of Chemistry, University of California, Irvine, California.

Current Protocols in Chemical Biology
|September 8, 2015
PubMed
Summary
This summary is machine-generated.

This study introduces novel virus electrodes for enhanced biosensing. These electrodes offer high-affinity biomarker detection and reagent-free, real-time measurements for cancer diagnostics.

Keywords:
click chemistryelectrochemical impedance spectroscopyphage displayphage wrappingprostate-specific membrane antigen

More Related Videos

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

17.9K
Bacterial Detection & Identification Using Electrochemical Sensors
09:30

Bacterial Detection & Identification Using Electrochemical Sensors

Published on: April 23, 2013

29.2K

Related Experiment Videos

Last Updated: Apr 4, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

12.7K
A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

17.9K
Bacterial Detection & Identification Using Electrochemical Sensors
09:30

Bacterial Detection & Identification Using Electrochemical Sensors

Published on: April 23, 2013

29.2K

Area of Science:

  • Biomaterials Science
  • Biosensor Technology
  • Nanotechnology

Background:

  • Biosensing faces challenges in specific biomarker binding and quantitative measurement.
  • Existing biosensors often require complex amplification steps and are not reagent-free.

Purpose of the Study:

  • To develop a novel biosensor using virus electrodes for enhanced biomarker detection.
  • To achieve high-affinity, quantitative, and real-time measurements of biomarkers.
  • To establish general protocols for creating modified virus biosensors.

Main Methods:

  • Tailoring virus surfaces for specific high-affinity binding to target biomarkers.
  • Entrapping modified viruses in a conducting polymer for electrical resistance-based detection.
  • Utilizing dual-ligand attachment on virus surfaces to increase binding avidity.

Main Results:

  • Achieved a 100 pM limit of detection for the cancer biomarker prostate-specific membrane antigen.
  • Demonstrated reagent-free, real-time quantitative measurements without enzymatic amplification.
  • Showcased the potential for enhanced detection of arbitrary target proteins.

Conclusions:

  • Virus electrodes offer a promising platform for sensitive and specific biosensing.
  • The dual-ligand approach significantly enhances biomarker detection sensitivity.
  • This method provides a versatile and efficient strategy for developing next-generation biosensors.