[Determining acebutolol level in the blood serum]

Area of Science:

• Analytical Chemistry
• Pharmacology

Context:

• Accurate quantification of acebutolol in biological samples is crucial for therapeutic drug monitoring and pharmacokinetic studies.
• Existing methods may lack sensitivity or require complex procedures.

Purpose:

• To develop a simple and sensitive method for the isolation and determination of acebutolol in blood serum.
• To establish conditions for thin-layer chromatography (TLC) for acebutolol detection.
• To validate the developed method using statistical analysis.

Summary:

• A straightforward method for isolating acebutolol from blood serum was established.
• Optimized thin-layer chromatography (TLC) conditions were defined for drug detection.
• A novel colorimetric method was developed for acebutolol determination following isolation and sulfuric acid hydrolysis.
• The method demonstrated a detection sensitivity of 0.5 micrograms for acebutolol via TLC.

Impact:

• Provides a practical and sensitive analytical technique for acebutolol quantification in clinical and research settings.
• Facilitates accurate therapeutic drug monitoring and pharmacokinetic research.
• The developed method offers a reliable alternative for acebutolol analysis in biological matrices.