Bio-coordination of bismuth in Helicobacter pylori revealed by immobilized metal affinity chromatography
Yuchuan Wang1, Cheuk-Nam Tsang1, Feng Xu2
1Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, P. R. China. hsun@hku.hk.
Abstract:
Over 300 Bi-binding peptides from 166 proteins in H. pylori were identified by Bi-IMAC. Bi(3+) exhibits high selectivity towards peptide enriched by cysteines and histidines with dominated motif patterns of CXnC, CXnH and HXnH. Structural rationalization and functional categorization on the identified Bi-binding peptides and proteins provide an insight into the inhibitory action of bismuth drugs.
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