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Updated: Apr 1, 2026

Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples
Published on: December 2, 2022
Real-time monitoring of rolling circle amplification using aggregation-induced emission: applications in biological
Hong-Xin Jiang1, Meng-Yao Zhao1, Chen-Di Niu1
1State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, 300071, People's Republic of China. kongdem@nankai.edu.cn and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300071, People's Republic of China.
Abstract:
We report that QATPE, an aggregation-induced emission-active tetraphenylethene dye, can be used as a non-sequence-specific ssDNA probe for real-time monitoring of all rolling circle amplification (RCA) reactions, thus making RCA more suitable for biosensing applications.
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