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Tamoxifen-Containing Eye Drops Successfully Trigger Cre-Mediated Recombination in the Entire Eye
Anja Schlecht1, Sarah V Leimbeck2, Ernst R Tamm3
1Institute of Human Anatomy and Embryology, University of Regensburg, Universitätsstr. 31, D-93053, Regensburg, Germany. Anja.Schlecht@vkl.uni-regensburg.de.
Abstract:
Embryonic lethality in mice with targeted gene deletion is a major issue that can be circumvented by using Cre-loxP-based animal models. Various inducible Cre systems are available, e.g. such that are activated following tamoxifen treatment, and allow deletion of a specific target gene at any desired time point during the life span of the animal. In this study, we describe the efficiency of topical tamoxifen administration by eye drops using a Cre- reporter mouse strain (R26R). We report that tamoxifen-responsive CAGGCre-ER (TM) mice show a robust Cre- mediated recombination throughout the entire eye.
Insights
Topical tamoxifen eye drops efficiently activate Cre-loxP systems in mouse eyes. This method allows precise gene deletion in ocular tissues, overcoming embryonic lethality challenges in genetic research.
Area of Science:
- Genetics and Genomics
- Developmental Biology
- Ophthalmology
Background:
- Embryonic lethality due to gene deletion in mice necessitates advanced genetic models.
- Cre-loxP systems offer temporal control for gene targeting, but efficient ocular delivery remains a challenge.
Purpose of the Study:
- To evaluate the efficacy of topical tamoxifen administration via eye drops for Cre-loxP-mediated gene deletion in the mouse eye.
- To assess the efficiency of recombination in ocular tissues using a tamoxifen-inducible Cre system.
Main Methods:
- Utilized a tamoxifen-responsive CAGGCre-ER (TM) mouse model crossed with a Cre-reporter strain (R26R).
- Administered tamoxifen solution as eye drops to induce Cre-mediated recombination.
- Analyzed recombination patterns throughout the entire eye structure.
Main Results:
- Topical eye drop administration of tamoxifen resulted in robust Cre-mediated recombination.
- Recombination was observed throughout the entire ocular structure, indicating efficient delivery and activation.
- This method successfully circumvents issues associated with systemic administration or embryonic lethality.
Conclusions:
- Topical tamoxifen eye drops provide an effective and targeted method for activating Cre-loxP systems in the mouse eye.
- This approach facilitates precise genetic manipulation in ocular tissues for research purposes.
- The study demonstrates a valuable tool for studying gene function in the eye and developing ocular therapies.
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