Characterization of Antibodies to Identify Cellular Expression of Dopamine Receptor 4
Janise D Deming1, Kathleen Van Craenenbroeck2, Yun Sung Eom3
1Departments of Ophthalmology, USC Eye Institute, Keck School of Medicine of the University of Southern California, Institute for Genetic Medicine, 2250 Alcazar St CSC 215, 90033, Los Angeles, CA, USA. jddeming@usc.edu.
Abstract:
The dopamine receptor D4 (DRD4) plays an important role in vision. In order to study the DRD4 expression in vivo, it is important to have antibodies that are specific for DRD4 for both immunoblot and immunohistochemical (IHC) applications. In this study, six antibodies raised against DRD4 peptides were tested in vitro, using transfected mammalian cells, and in vivo, using mouse retinas. Three Santa Cruz (SC) antibodies, D-16, N-20, and R-20, were successful in IHC of transfected DRD4; however, N-20 was the only one effective on immunoblot analysis in DRD4 transfected cells and IHC of mouse retinal sections, while R-20, 2B9, and Antibody Verify AAS63631C were non-specific or below detection.
