Common Non-classically Secreted Bacterial Proteins with Experimental Evidence

Area of Science:

• Microbiology
• Molecular Biology
• Biochemistry

Background:

• Non-classically secreted proteins lack signal peptides for extracellular localization, posing research challenges.
• Existing studies show inconsistencies, complicating the identification of these proteins.
• Artefactual contamination from sample preparation can further hinder accurate identification.

Purpose of the Study:

• To compile a reliable list of commonly identified non-classically secreted proteins.
• To address inconsistencies in current research findings.
• To explore the potential of non-classical secretion for recombinant protein production.

Main Methods:

• Literature review compiling data from various research groups.
• Cross-referencing studies across different bacterial species.
• Analysis of protein secretion pathways and potential contamination sources.

Main Results:

• A curated list of 45 commonly identified non-classically secreted proteins was established.
• The compiled list reduces the impact of experimental artifacts.
• Identified proteins serve as reliable experimental material for further studies.

Conclusions:

• The compiled list offers a foundation for advancing research in non-classical protein secretion.
• Certain non-classically secreted proteins show hyper-secretion potential under specific conditions.
• The non-classical secretion pathway presents opportunities for recombinant protein production.