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Updated: Apr 1, 2026
12:04
Mouse Genome Engineering Using Designer Nucleases
Published on: April 2, 2014
29.4K
The Development of TALE Nucleases for Biotechnology
David G Ousterout1, Charles A Gersbach2,3,4
1Department of Biomedical Engineering, Duke University, Durham, NC, 27708, USA.
Methods in Molecular Biology (Clifton, N.J.)
|October 8, 2015
Summary
Transcription activator-like effector nucleases (TALENs) offer a powerful genome engineering tool. This review covers TALEN development, applications in basic science, biotechnology, and medicine, including gene therapy and disease modeling.
Area of Science:
- Molecular Biology
- Biotechnology
- Genetics
Background:
- Transcription activator-like effectors (TALEs) are DNA-binding proteins.
- TALENs combine TALE DNA-binding domains with nuclease domains for targeted genome modification.
Purpose of the Study:
- To provide a comprehensive review of TALEN technology.
- To discuss the development, applications, and future directions of TALENs in science and medicine.
Main Methods:
- Review of scientific literature on TALENs.
- Discussion of TALE DNA recognition and protein engineering.
- Analysis of nuclease-based editing strategies.
Main Results:
- TALENs enable precise genome engineering across various fields.
- Applications include studying gene function, creating disease models, and developing gene therapies.
- Challenges and future prospects of TALEN technology are highlighted.
Conclusions:
- TALENs represent a significant advancement in genome engineering.
- The technology has broad implications for basic research, biotechnology, and therapeutic development.
- Continued innovation promises expanded applications for TALENs.
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