Related Experiment Video
Updated: Mar 31, 2026
Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis
Published on: September 7, 2021
Can Comprehensive Chromosome Screening Technology Improve IVF/ICSI Outcomes? A Meta-Analysis
Minghao Chen1, Shiyou Wei2, Junyan Hu3
1Department of Obstetrics and Gynecology, Reproductive Centre, Nanfang Hospital, Southern Medical University, Guangzhou, China.
Comprehensive chromosome screening (CCS) for preimplantation genetic screening (PGS) improves in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) outcomes compared to traditional methods. CCS-euploid embryo transfer enhances implantation rates, offering better results for patients undergoing fertility treatments.
Area of Science:
- Reproductive medicine
- Genetics
- Embryology
Background:
- Traditional methods for embryo selection in IVF/ICSI rely on morphological assessment.
- Preimplantation genetic screening (PGS) aims to improve IVF/ICSI success rates by identifying chromosomally normal embryos.
- Comprehensive chromosome screening (CCS) is an advanced PGS technique evaluating all chromosomes.
Purpose of the Study:
- To evaluate the effectiveness of CCS for PGS in improving IVF/ICSI outcomes compared to traditional morphological assessment.
- To determine if CCS-based embryo selection leads to better implantation, pregnancy, and live birth rates.
Main Methods:
- A systematic literature search was performed across major databases (PubMed, EMBASE, CNKI, ClinicalTrials.gov) up to May 2015.
- Included studies compared IVF/ICSI outcomes between CCS-based embryo selection and traditional morphological methods.
- Meta-analysis, subgroup analysis, and Begg's test were employed to assess outcomes, heterogeneity, and publication bias.
Main Results:
- Meta-analysis of 4 RCTs and 7 cohort studies showed CCS-identified euploid embryos had higher implantation rates (RCT RR 1.32; cohort RR 1.74).
- CCS-based PGS was associated with increased clinical pregnancy (cohort RR 1.48), ongoing pregnancy (cohort RR 1.61), and live birth rates (RCT RR 1.26; cohort RR 1.35).
- CCS also demonstrated reduced miscarriage rates (cohort RR 0.31) and multiple pregnancy rates (RCT RR 0.02; cohort RR 0.19).
Conclusions:
- CCS-based PGS is effective and comparable to traditional methods, yielding superior outcomes for IVF/ICSI patients.
- Transferring CCS-euploid embryos, regardless of biopsy method (trophectoderm or blastomere), significantly improves implantation rates.
