A course-based undergraduate research experience investigating p300 bromodomain mutations

Area of Science:

• Molecular Biology
• Genetics
• Biochemistry

Background:

• Course-based undergraduate research experiences (CUREs) integrate research into coursework.
• Traditional research experiences are limited in scale.
• The p300 protein's bromodomain regulates gene transcription via histone acetylation.

Purpose of the Study:

• To investigate the impact of clinically relevant mutations in the p300 bromodomain on its interaction with acetylated histones.
• To provide authentic research experiences for a large number of undergraduates.
• To bridge the gap between classroom learning and laboratory research.

Main Methods:

• Students utilized the Catalogue of Somatic Mutations in Cancer (COSMIC) database to identify mutations.
• Cloning and purification of mutated p300 bromodomain constructs.
• Peptide pulldown assays were performed to assess binding to acetylated histones.

Main Results:

• Students gained confidence in molecular techniques.
• Positive student feedback on the research project.
• Generated data were validated in a research laboratory setting.

Conclusions:

• CUREs can effectively train students in molecular biology techniques and research principles.
• This CURE model successfully generated valuable scientific data.
• The approach facilitates faculty engagement in both course-based and individual research.